Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides

We report that engineered Cas9 variants with improved specificity—eCas9-1.1 and Cas9-HF1—are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5’ terminus, relative to target DNA sequences. Because the nucleotide at the 5’ end of sgRNAs, expres...

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Detalles Bibliográficos
Autores principales: Kim, Sojung, Bae, Taegeun, Hwang, Jaewoong, Kim, Jin-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686910/
https://www.ncbi.nlm.nih.gov/pubmed/29141659
http://dx.doi.org/10.1186/s13059-017-1355-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686910/
https://www.ncbi.nlm.nih.gov/pubmed/29141659
http://dx.doi.org/10.1186/s13059-017-1355-3

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