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indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events

Genetic manipulation of organisms using CRISPR/Cas9 technology generally produces small insertions/deletions (indels) that can be difficult to detect. Here, we describe a technique to easily and rapidly identify such indels. Sequence-identified mutations that alter a restriction enzyme recognition s...

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Detalles Bibliográficos
Autores principales: Hodgens, Charles, Nimchuk, Zachary L., Kieber, Joseph J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5687723/
https://www.ncbi.nlm.nih.gov/pubmed/29141013
http://dx.doi.org/10.1371/journal.pone.0188406