SensorFRET: A Standardless Approach to Measuring Pixel-based Spectral Bleed-through and FRET Efficiency using Spectral Imaging

Fluorescence microscopy of FRET-based biosensors allow nanoscale interactions to be probed in living cells. This paper describes a novel approach to spectrally resolved fluorescence microscopy, termed sensorFRET, that enables quantitative measurement of FRET efficiency. This approach is an improveme...

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Detalles Bibliográficos
Autores principales: Arsenovic, Paul T., Mayer, Carl R., Conway, Daniel E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688180/
https://www.ncbi.nlm.nih.gov/pubmed/29142199
http://dx.doi.org/10.1038/s41598-017-15411-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688180/
https://www.ncbi.nlm.nih.gov/pubmed/29142199
http://dx.doi.org/10.1038/s41598-017-15411-8

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