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Steps to achieve quantitative measurements of microRNA using two step droplet digital PCR

Droplet digital PCR (ddPCR) is being advocated as a reference method to measure rare genomic targets. It has consistently been proven to be more sensitive and direct at discerning copy numbers of DNA than other quantitative methods. However, one of the largest obstacles to measuring microRNA (miRNA)...

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Detalles Bibliográficos
Autores principales: Stein, Erica V., Duewer, David L., Farkas, Natalia, Romsos, Erica L., Wang, Lili, Cole, Kenneth D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5690473/
https://www.ncbi.nlm.nih.gov/pubmed/29145448
http://dx.doi.org/10.1371/journal.pone.0188085