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Steps to achieve quantitative measurements of microRNA using two step droplet digital PCR
Droplet digital PCR (ddPCR) is being advocated as a reference method to measure rare genomic targets. It has consistently been proven to be more sensitive and direct at discerning copy numbers of DNA than other quantitative methods. However, one of the largest obstacles to measuring microRNA (miRNA)...
Autores principales: | Stein, Erica V., Duewer, David L., Farkas, Natalia, Romsos, Erica L., Wang, Lili, Cole, Kenneth D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5690473/ https://www.ncbi.nlm.nih.gov/pubmed/29145448 http://dx.doi.org/10.1371/journal.pone.0188085 |
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