Automated Solid-Phase Protein Modification with Integrated Enzymatic Digest for Reaction Validation: Application of a Compartmented Microfluidic Reactor for Rapid Optimization and Analysis of Protein Biotinylation

Protein modification by covalent coupling of small ligands or markers is an important prerequisite for the use of proteins in many applications. Well-known examples are the use of proteins with fluorescent markers in many in vivo experiments or the binding of biotinylated antibodies via biotin–strep...

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Detalles Bibliográficos
Autores principales: Fraas, Regina, Diehm, Juliane, Franzreb, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693853/
https://www.ncbi.nlm.nih.gov/pubmed/29181376
http://dx.doi.org/10.3389/fbioe.2017.00072

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693853/
https://www.ncbi.nlm.nih.gov/pubmed/29181376
http://dx.doi.org/10.3389/fbioe.2017.00072

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