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A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris
Although recent advances in E. coli self-assembly have greatly simplified cloning, these have not yet been harnessed for the high-throughput generation of expression strains in the early research and discovery phases of biopharmaceutical production. Here, we have refined the technique and incorporat...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693959/ https://www.ncbi.nlm.nih.gov/pubmed/29150665 http://dx.doi.org/10.1038/s41598-017-16172-0 |