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Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease

The aim of this study was to optimise and evaluate an intracellular cytokine staining (ICS) assay for assessment of T cell IFN-γ responses in chickens vaccinated against Newcastle disease (ND). We aimed to validate currently available antibodies to chicken IFN-γ using transfected CHO cells. Moreover...

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Detalles Bibliográficos
Autores principales: Andersen, S.H., Vervelde, L., Sutton, K., Norup, L.R., Wattrang, E., Juul-Madsen, H.R., Dalgaard, T.S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Scientific 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697524/
https://www.ncbi.nlm.nih.gov/pubmed/29129224
http://dx.doi.org/10.1016/j.vetimm.2017.10.001