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Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease
The aim of this study was to optimise and evaluate an intracellular cytokine staining (ICS) assay for assessment of T cell IFN-γ responses in chickens vaccinated against Newcastle disease (ND). We aimed to validate currently available antibodies to chicken IFN-γ using transfected CHO cells. Moreover...
Autores principales: | Andersen, S.H., Vervelde, L., Sutton, K., Norup, L.R., Wattrang, E., Juul-Madsen, H.R., Dalgaard, T.S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Scientific
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5697524/ https://www.ncbi.nlm.nih.gov/pubmed/29129224 http://dx.doi.org/10.1016/j.vetimm.2017.10.001 |
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