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RNA‐guided Cas9 as an in vivo desired‐target mutator in maize

The RNA‐guided Cas9 system is a versatile tool for genome editing. Here, we established a RNA‐guided endonuclease (RGEN) system as an in vivo desired‐target mutator (DTM) in maize to reduce the linkage drag during breeding procedure, using the LIGULELESS1 (LG1) locus as a proof‐of‐concept. Our syste...

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Detalles Bibliográficos
Autores principales: Li, Chuxi, Liu, Changlin, Qi, Xiantao, Wu, Yongchun, Fei, Xiaohong, Mao, Long, Cheng, Beijiu, Li, Xinhai, Xie, Chuanxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5698053/
https://www.ncbi.nlm.nih.gov/pubmed/28379609
http://dx.doi.org/10.1111/pbi.12739