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RNA‐guided Cas9 as an in vivo desired‐target mutator in maize
The RNA‐guided Cas9 system is a versatile tool for genome editing. Here, we established a RNA‐guided endonuclease (RGEN) system as an in vivo desired‐target mutator (DTM) in maize to reduce the linkage drag during breeding procedure, using the LIGULELESS1 (LG1) locus as a proof‐of‐concept. Our syste...
Autores principales: | Li, Chuxi, Liu, Changlin, Qi, Xiantao, Wu, Yongchun, Fei, Xiaohong, Mao, Long, Cheng, Beijiu, Li, Xinhai, Xie, Chuanxiao |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5698053/ https://www.ncbi.nlm.nih.gov/pubmed/28379609 http://dx.doi.org/10.1111/pbi.12739 |
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