Characterizing a thermostable Cas9 for bacterial genome editing and silencing

CRISPR-Cas9-based genome engineering tools have revolutionized fundamental research and biotechnological exploitation of both eukaryotes and prokaryotes. However, the mesophilic nature of the established Cas9 systems does not allow for applications that require enhanced stability, including engineer...

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Detalles Bibliográficos
Autores principales: Mougiakos, Ioannis, Mohanraju, Prarthana, Bosma, Elleke F., Vrouwe, Valentijn, Finger Bou, Max, Naduthodi, Mihris I. S., Gussak, Alex, Brinkman, Rudolf B. L., van Kranenburg, Richard, van der Oost, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5698299/
https://www.ncbi.nlm.nih.gov/pubmed/29162801
http://dx.doi.org/10.1038/s41467-017-01591-4