Phospholipase A2-Responsive Phosphate Micelle-Loaded UCNPs for Bioimaging of Prostate Cancer Cells

We report the effective synthesis of biocompatible upconversion nanoparticles (UCNP)-loaded phosphate micelles and successful delivery of UCNPs to prostate cancer cells via secreted phospholipase A2 (sPLA-2) enzyme cleavage of the loaded micelles for the first time. The activity of the (sPLA-2) enzy...

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Autores principales: Sharipov, Mirkomil, Tawfik, Salah M., Gerelkhuu, Zayakhuu, Huy, Bui The, Lee, Yong-Ill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700164/
https://www.ncbi.nlm.nih.gov/pubmed/29167526
http://dx.doi.org/10.1038/s41598-017-16136-4
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author Sharipov, Mirkomil
Tawfik, Salah M.
Gerelkhuu, Zayakhuu
Huy, Bui The
Lee, Yong-Ill
author_facet Sharipov, Mirkomil
Tawfik, Salah M.
Gerelkhuu, Zayakhuu
Huy, Bui The
Lee, Yong-Ill
author_sort Sharipov, Mirkomil
collection PubMed
description We report the effective synthesis of biocompatible upconversion nanoparticles (UCNP)-loaded phosphate micelles and successful delivery of UCNPs to prostate cancer cells via secreted phospholipase A2 (sPLA-2) enzyme cleavage of the loaded micelles for the first time. The activity of the (sPLA-2) enzyme toward the synthesized micelles was investigated and confirmed by LC-MS. TEM results showed that the micelles have a size distribution of 80 to 150 nm, whereas UCNP-loaded micelles range from 200 to 350 nm, indicating the successful loading of UCNPs. The selective release of UCNPs to prostate cancer cells rather than other cells, specifically cervical cancer cells, was observed and confirmed by a range of bioimaging studies. Moreover, cytotoxicity assays confirmed the biocompatibility of the UCNP-loaded micelles.
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spelling pubmed-57001642017-11-30 Phospholipase A2-Responsive Phosphate Micelle-Loaded UCNPs for Bioimaging of Prostate Cancer Cells Sharipov, Mirkomil Tawfik, Salah M. Gerelkhuu, Zayakhuu Huy, Bui The Lee, Yong-Ill Sci Rep Article We report the effective synthesis of biocompatible upconversion nanoparticles (UCNP)-loaded phosphate micelles and successful delivery of UCNPs to prostate cancer cells via secreted phospholipase A2 (sPLA-2) enzyme cleavage of the loaded micelles for the first time. The activity of the (sPLA-2) enzyme toward the synthesized micelles was investigated and confirmed by LC-MS. TEM results showed that the micelles have a size distribution of 80 to 150 nm, whereas UCNP-loaded micelles range from 200 to 350 nm, indicating the successful loading of UCNPs. The selective release of UCNPs to prostate cancer cells rather than other cells, specifically cervical cancer cells, was observed and confirmed by a range of bioimaging studies. Moreover, cytotoxicity assays confirmed the biocompatibility of the UCNP-loaded micelles. Nature Publishing Group UK 2017-11-22 /pmc/articles/PMC5700164/ /pubmed/29167526 http://dx.doi.org/10.1038/s41598-017-16136-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sharipov, Mirkomil
Tawfik, Salah M.
Gerelkhuu, Zayakhuu
Huy, Bui The
Lee, Yong-Ill
Phospholipase A2-Responsive Phosphate Micelle-Loaded UCNPs for Bioimaging of Prostate Cancer Cells
title Phospholipase A2-Responsive Phosphate Micelle-Loaded UCNPs for Bioimaging of Prostate Cancer Cells
title_full Phospholipase A2-Responsive Phosphate Micelle-Loaded UCNPs for Bioimaging of Prostate Cancer Cells
title_fullStr Phospholipase A2-Responsive Phosphate Micelle-Loaded UCNPs for Bioimaging of Prostate Cancer Cells
title_full_unstemmed Phospholipase A2-Responsive Phosphate Micelle-Loaded UCNPs for Bioimaging of Prostate Cancer Cells
title_short Phospholipase A2-Responsive Phosphate Micelle-Loaded UCNPs for Bioimaging of Prostate Cancer Cells
title_sort phospholipase a2-responsive phosphate micelle-loaded ucnps for bioimaging of prostate cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700164/
https://www.ncbi.nlm.nih.gov/pubmed/29167526
http://dx.doi.org/10.1038/s41598-017-16136-4
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