Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

Since its initial application in mammalian cells, CRISPR-Cas9 has rapidly become a preferred method for genome engineering experiments. The Cas9 nuclease is targeted to genomic DNA using guide RNAs (gRNA), either as the native dual RNA system consisting of a DNA-targeting CRISPR RNA (crRNA) and a tr...

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Detalles Bibliográficos
Autores principales: Basila, Megan, Kelley, Melissa L., Smith, Anja van Brabant
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703482/
https://www.ncbi.nlm.nih.gov/pubmed/29176845
http://dx.doi.org/10.1371/journal.pone.0188593

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703482/
https://www.ncbi.nlm.nih.gov/pubmed/29176845
http://dx.doi.org/10.1371/journal.pone.0188593

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