Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT

Conventional peptide-major histocompatibility complex (pMHC) multimer staining, intracellular cytokine staining, and enzyme-linked immunospot (ELISPOT) assay cannot concurrently determine the frequency and reactivity of antigen-specific T cells (AST) in a single assay. In this report, pMHC multimer,...

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Detalles Bibliográficos
Autores principales: Shen, Chuanlai, Xu, Tao, Wu, You, Li, Xiaoe, Xia, Lingzhi, Wang, Wei, Shahzad, Khawar Ali, Zhang, Lei, Wan, Xin, Qiu, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703716/
https://www.ncbi.nlm.nih.gov/pubmed/29180767
http://dx.doi.org/10.1038/s41598-017-16549-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703716/
https://www.ncbi.nlm.nih.gov/pubmed/29180767
http://dx.doi.org/10.1038/s41598-017-16549-1

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