Rational design of cholesterol oxidase for efficient bioresolution of cholestane skeleton substrates

Cholesterol oxidase catalyzes the oxidation and isomerization of the cholestane substrates leading to the addition of a hydroxyl group at the C3 position. Rational engineering of the cholesterol oxidase from Pimelobacter simplex (PsChO) was performed. Mutagenesis of V64 and F70 improved the catalyti...

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Detalles Bibliográficos
Autores principales: Qin, Hui-Min, Zhu, Zhangliang, Ma, Zheng, Xu, Panpan, Guo, Qianqian, Li, Songtao, Wang, Jian-Wen, Mao, Shuhong, Liu, Fufeng, Lu, Fuping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703901/
https://www.ncbi.nlm.nih.gov/pubmed/29180806
http://dx.doi.org/10.1038/s41598-017-16768-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5703901/
https://www.ncbi.nlm.nih.gov/pubmed/29180806
http://dx.doi.org/10.1038/s41598-017-16768-6

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