Gene-Based Pathogen Detection: Can We Use qPCR to Predict the Outcome of Diagnostic Metagenomics?

In microbial food safety, molecular methods such as quantitative PCR (qPCR) and next-generation sequencing (NGS) of bacterial isolates can potentially be replaced by diagnostic shotgun metagenomics. However, the methods for pre-analytical sample preparation are often optimized for qPCR, and do not n...

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Detalles Bibliográficos
Autores principales: Andersen, Sandra Christine, Fachmann, Mette Sofie Rousing, Kiil, Kristoffer, Møller Nielsen, Eva, Hoorfar, Jeffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704245/
https://www.ncbi.nlm.nih.gov/pubmed/29156625
http://dx.doi.org/10.3390/genes8110332

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704245/
https://www.ncbi.nlm.nih.gov/pubmed/29156625
http://dx.doi.org/10.3390/genes8110332

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