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Quantitative, in situ analysis of mRNAs and proteins with subcellular resolution

We describe here a method, termed immunoFISH, for simultaneous in situ analysis of the composition and distribution of proteins and individual RNA transcripts in single cells. Individual RNA molecules are labeled by hybridization and target proteins are concurrently stained using immunofluorescence....

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Detalles Bibliográficos
Autores principales: Kwon, Sunjong, Chin, Koei, Nederlof, Michel, Gray, Joe W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705767/
https://www.ncbi.nlm.nih.gov/pubmed/29184166
http://dx.doi.org/10.1038/s41598-017-16492-1