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Quantitative, in situ analysis of mRNAs and proteins with subcellular resolution
We describe here a method, termed immunoFISH, for simultaneous in situ analysis of the composition and distribution of proteins and individual RNA transcripts in single cells. Individual RNA molecules are labeled by hybridization and target proteins are concurrently stained using immunofluorescence....
Autores principales: | Kwon, Sunjong, Chin, Koei, Nederlof, Michel, Gray, Joe W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705767/ https://www.ncbi.nlm.nih.gov/pubmed/29184166 http://dx.doi.org/10.1038/s41598-017-16492-1 |
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