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The Natural Carotenoid Crocetin and the Synthetic Tellurium Compound AS101 Protect the Ovary against Cyclophosphamide by Modulating SIRT1 and Mitochondrial Markers

Cancer therapies are associated with increased infertility risk due to accelerated reproductive aging. Oxidative stress (OS) is a potential mechanism behind ovarian toxicity by cyclophosphamide (CPM), the most ovotoxic anticancer drug. An important sensor of OS is SIRT1, a NAD(+)-dependent deacetyla...

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Autores principales: Di Emidio, Giovanna, Rossi, Giulia, Bonomo, Isabelle, Alonso, Gonzalo Luis, Sferra, Roberta, Vetuschi, Antonella, Artini, Paolo Giovanni, Provenzani, Alessandro, Falone, Stefano, Carta, Gaspare, D'Alessandro, Anna Maria, Amicarelli, Fernanda, Tatone, Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705900/
https://www.ncbi.nlm.nih.gov/pubmed/29270246
http://dx.doi.org/10.1155/2017/8928604
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author Di Emidio, Giovanna
Rossi, Giulia
Bonomo, Isabelle
Alonso, Gonzalo Luis
Sferra, Roberta
Vetuschi, Antonella
Artini, Paolo Giovanni
Provenzani, Alessandro
Falone, Stefano
Carta, Gaspare
D'Alessandro, Anna Maria
Amicarelli, Fernanda
Tatone, Carla
author_facet Di Emidio, Giovanna
Rossi, Giulia
Bonomo, Isabelle
Alonso, Gonzalo Luis
Sferra, Roberta
Vetuschi, Antonella
Artini, Paolo Giovanni
Provenzani, Alessandro
Falone, Stefano
Carta, Gaspare
D'Alessandro, Anna Maria
Amicarelli, Fernanda
Tatone, Carla
author_sort Di Emidio, Giovanna
collection PubMed
description Cancer therapies are associated with increased infertility risk due to accelerated reproductive aging. Oxidative stress (OS) is a potential mechanism behind ovarian toxicity by cyclophosphamide (CPM), the most ovotoxic anticancer drug. An important sensor of OS is SIRT1, a NAD(+)-dependent deacetylase which regulates cellular defence and cell fate. This study investigated whether the natural carotenoid crocetin and the synthetic compound AS101 protect the ovary against CPM by modulating SIRT1 and mitochondrial markers. We found that the number of primordial follicles of female CD1 mice receiving crocetin plus CPM increased when compared with CPM alone and similar to AS101, whose protective effects are known. SIRT1 increased in CPM mouse ovaries revealing the occurrence of OS. Similarly, mitochondrial SIRT3 rose, whilst SOD2 and the mitochondrial biogenesis activator PGC1-α decreased, suggesting the occurrence of mitochondrial damage. Crocetin and AS101 administration prevented SIRT1 burst suggesting that preservation of redox balance can help the ovary to counteract ovarian damage by CPM. Decreased SIRT3 and increased SOD2 and PGC1-α in mice receiving crocetin or AS101 prior to CPM provide evidence for mitochondrial protection. Present results improve the knowledge of ovarian damage by CPM and may help to develop interventions for preserving fertility in cancer patients.
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spelling pubmed-57059002017-12-21 The Natural Carotenoid Crocetin and the Synthetic Tellurium Compound AS101 Protect the Ovary against Cyclophosphamide by Modulating SIRT1 and Mitochondrial Markers Di Emidio, Giovanna Rossi, Giulia Bonomo, Isabelle Alonso, Gonzalo Luis Sferra, Roberta Vetuschi, Antonella Artini, Paolo Giovanni Provenzani, Alessandro Falone, Stefano Carta, Gaspare D'Alessandro, Anna Maria Amicarelli, Fernanda Tatone, Carla Oxid Med Cell Longev Research Article Cancer therapies are associated with increased infertility risk due to accelerated reproductive aging. Oxidative stress (OS) is a potential mechanism behind ovarian toxicity by cyclophosphamide (CPM), the most ovotoxic anticancer drug. An important sensor of OS is SIRT1, a NAD(+)-dependent deacetylase which regulates cellular defence and cell fate. This study investigated whether the natural carotenoid crocetin and the synthetic compound AS101 protect the ovary against CPM by modulating SIRT1 and mitochondrial markers. We found that the number of primordial follicles of female CD1 mice receiving crocetin plus CPM increased when compared with CPM alone and similar to AS101, whose protective effects are known. SIRT1 increased in CPM mouse ovaries revealing the occurrence of OS. Similarly, mitochondrial SIRT3 rose, whilst SOD2 and the mitochondrial biogenesis activator PGC1-α decreased, suggesting the occurrence of mitochondrial damage. Crocetin and AS101 administration prevented SIRT1 burst suggesting that preservation of redox balance can help the ovary to counteract ovarian damage by CPM. Decreased SIRT3 and increased SOD2 and PGC1-α in mice receiving crocetin or AS101 prior to CPM provide evidence for mitochondrial protection. Present results improve the knowledge of ovarian damage by CPM and may help to develop interventions for preserving fertility in cancer patients. Hindawi 2017 2017-11-15 /pmc/articles/PMC5705900/ /pubmed/29270246 http://dx.doi.org/10.1155/2017/8928604 Text en Copyright © 2017 Giovanna Di Emidio et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Di Emidio, Giovanna
Rossi, Giulia
Bonomo, Isabelle
Alonso, Gonzalo Luis
Sferra, Roberta
Vetuschi, Antonella
Artini, Paolo Giovanni
Provenzani, Alessandro
Falone, Stefano
Carta, Gaspare
D'Alessandro, Anna Maria
Amicarelli, Fernanda
Tatone, Carla
The Natural Carotenoid Crocetin and the Synthetic Tellurium Compound AS101 Protect the Ovary against Cyclophosphamide by Modulating SIRT1 and Mitochondrial Markers
title The Natural Carotenoid Crocetin and the Synthetic Tellurium Compound AS101 Protect the Ovary against Cyclophosphamide by Modulating SIRT1 and Mitochondrial Markers
title_full The Natural Carotenoid Crocetin and the Synthetic Tellurium Compound AS101 Protect the Ovary against Cyclophosphamide by Modulating SIRT1 and Mitochondrial Markers
title_fullStr The Natural Carotenoid Crocetin and the Synthetic Tellurium Compound AS101 Protect the Ovary against Cyclophosphamide by Modulating SIRT1 and Mitochondrial Markers
title_full_unstemmed The Natural Carotenoid Crocetin and the Synthetic Tellurium Compound AS101 Protect the Ovary against Cyclophosphamide by Modulating SIRT1 and Mitochondrial Markers
title_short The Natural Carotenoid Crocetin and the Synthetic Tellurium Compound AS101 Protect the Ovary against Cyclophosphamide by Modulating SIRT1 and Mitochondrial Markers
title_sort natural carotenoid crocetin and the synthetic tellurium compound as101 protect the ovary against cyclophosphamide by modulating sirt1 and mitochondrial markers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705900/
https://www.ncbi.nlm.nih.gov/pubmed/29270246
http://dx.doi.org/10.1155/2017/8928604
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