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Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures
BACKGROUND: Escherichia coli (E. coli) is the most abundant expression host for recombinant proteins. The production efficiency is dependent on a multitude of parameters. Therefore, high-throughput applications have become an increasingly frequent technique to investigate the main factors. Within th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706349/ https://www.ncbi.nlm.nih.gov/pubmed/29183374 http://dx.doi.org/10.1186/s12934-017-0832-4 |