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Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures

BACKGROUND: Escherichia coli (E. coli) is the most abundant expression host for recombinant proteins. The production efficiency is dependent on a multitude of parameters. Therefore, high-throughput applications have become an increasingly frequent technique to investigate the main factors. Within th...

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Detalles Bibliográficos
Autores principales:	Mühlmann, Martina, Forsten, Eva, Noack, Saskia, Büchs, Jochen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706349/ https://www.ncbi.nlm.nih.gov/pubmed/29183374 http://dx.doi.org/10.1186/s12934-017-0832-4

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