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Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release

BACKGROUND: The development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (Panicum virgatum). Switchgrass is an outcrossing species with an allotetraploid genome (2n = 4x = 36), a complexity which forms an impediment to generating homozygous knock-...

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Detalles Bibliográficos
Autores principales: Park, Jong-Jin, Yoo, Chang Geun, Flanagan, Amy, Pu, Yunqiao, Debnath, Smriti, Ge, Yaxin, Ragauskas, Arthur J., Wang, Zeng-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708096/
https://www.ncbi.nlm.nih.gov/pubmed/29213323
http://dx.doi.org/10.1186/s13068-017-0972-0