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Traceless Cleavage of Protein–Biotin Conjugates under Biologically Compatible Conditions

Biotinylation of amines is widely used to conjugate biomolecules, but either the resulting label is non‐removable or its removal leaves a tag on the molecule of interest, thus affecting downstream processes. We present here a set of reagents (RevAmines) that allow traceless, reversible biotinylation...

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Detalles Bibliográficos
Autores principales: Cowell, Joseph, Buck, Matthew, Essa, Ali H., Clarke, Rebecca, Vollmer, Waldemar, Vollmer, Daniela, Hilkens, Catharien M., Isaacs, John D., Hall, Michael J., Gray, Joe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708275/
https://www.ncbi.nlm.nih.gov/pubmed/28581639
http://dx.doi.org/10.1002/cbic.201700214