Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA

Oligonucleotides containing internal triazole–3′-LNA linkages bind to complementary RNA with similar affinity and specificity to unmodified oligonucleotides, and significantly better than oligonucleotides containing triazole alone. In contrast LNA on the 5′-side of the triazole does not stabilise du...

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Detalles Bibliográficos
Autores principales: Kumar, Pawan, El-Sagheer, Afaf H., Truong, Lynda, Brown, Tom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708354/
https://www.ncbi.nlm.nih.gov/pubmed/28748236
http://dx.doi.org/10.1039/c7cc05159j
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author Kumar, Pawan
El-Sagheer, Afaf H.
Truong, Lynda
Brown, Tom
author_facet Kumar, Pawan
El-Sagheer, Afaf H.
Truong, Lynda
Brown, Tom
author_sort Kumar, Pawan
collection PubMed
description Oligonucleotides containing internal triazole–3′-LNA linkages bind to complementary RNA with similar affinity and specificity to unmodified oligonucleotides, and significantly better than oligonucleotides containing triazole alone. In contrast LNA on the 5′-side of the triazole does not stabilise duplexes. Triazole–LNA confers great resistance towards enzymatic degradation relative to LNA alone.
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spelling pubmed-57083542018-01-05 Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA Kumar, Pawan El-Sagheer, Afaf H. Truong, Lynda Brown, Tom Chem Commun (Camb) Chemistry Oligonucleotides containing internal triazole–3′-LNA linkages bind to complementary RNA with similar affinity and specificity to unmodified oligonucleotides, and significantly better than oligonucleotides containing triazole alone. In contrast LNA on the 5′-side of the triazole does not stabilise duplexes. Triazole–LNA confers great resistance towards enzymatic degradation relative to LNA alone. Royal Society of Chemistry 2017-08-14 2017-07-27 /pmc/articles/PMC5708354/ /pubmed/28748236 http://dx.doi.org/10.1039/c7cc05159j Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Kumar, Pawan
El-Sagheer, Afaf H.
Truong, Lynda
Brown, Tom
Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA
title Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA
title_full Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA
title_fullStr Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA
title_full_unstemmed Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA
title_short Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA
title_sort locked nucleic acid (lna) enhances binding affinity of triazole-linked dna towards rna
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708354/
https://www.ncbi.nlm.nih.gov/pubmed/28748236
http://dx.doi.org/10.1039/c7cc05159j
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AT browntom lockednucleicacidlnaenhancesbindingaffinityoftriazolelinkeddnatowardsrna

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