CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency

The CRISPR/Cas9 system is a powerful, revolutionary tool for genome editing. However, it is not without limitations. There are PAM-free and CRISPR-tolerant regions that cannot be modified by the standard CRISPR/Cas9 system, and off-target activity impedes its broader applications. To avoid these dra...

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Detalles Bibliográficos
Autores principales: Zhao, Dongdong, Feng, Xu, Zhu, Xinna, Wu, Tao, Zhang, Xueli, Bi, Changhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709385/
https://www.ncbi.nlm.nih.gov/pubmed/29192199
http://dx.doi.org/10.1038/s41598-017-16998-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709385/
https://www.ncbi.nlm.nih.gov/pubmed/29192199
http://dx.doi.org/10.1038/s41598-017-16998-8

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