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Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose
Chronic exposure of pancreatic β-cells to high glucose levels results in β-cell dysfunction and death. These effects can be protected by estrogen. The local pancreatic renin-angiotensin system (RAS) has been shown as a novel pathological pathway of high-glucose-induced cell death. The effect of estr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709427/ https://www.ncbi.nlm.nih.gov/pubmed/29192236 http://dx.doi.org/10.1038/s41598-017-15237-4 |
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author | Kooptiwut, Suwattanee Wanchai, Keerati Semprasert, Namoiy Srisawat, Chatchawan Yenchitsomanus, Pa-thai |
author_facet | Kooptiwut, Suwattanee Wanchai, Keerati Semprasert, Namoiy Srisawat, Chatchawan Yenchitsomanus, Pa-thai |
author_sort | Kooptiwut, Suwattanee |
collection | PubMed |
description | Chronic exposure of pancreatic β-cells to high glucose levels results in β-cell dysfunction and death. These effects can be protected by estrogen. The local pancreatic renin-angiotensin system (RAS) has been shown as a novel pathological pathway of high-glucose-induced cell death. The effect of estrogen on pancreatic RAS is still unknown. This study examines whether estrogen protects against pancreatic β-cell death caused by glucotoxicity via a decrease in the pancreatic β-cell RAS pathway. When INS-1 cells were cultured in a high glucose medium, cell death was significantly higher than when the cells were cultured in a basal glucose medium; similarly, there were also higher levels of AGTR1 and p47 (ph) ° (x) mRNA, and protein expression. Moreover, the addition of 10(−8) M 17β-estradiol to INS-1 cells cultured in a high glucose medium markedly reduced cell death, AGTR1 and p47 (ph) ° (x) mRNA levels, and protein expression. Similar results were demonstrated in the pancreatic islets. The presence of 10(−8) M 17β-estradiol, losartan, or a combination of both, in a high glucose medium had similar levels of reduction of p47 (ph) ° (x) mRNA and protein expression, compared with those cultured in high glucose. Taken together, estrogen protected pancreatic β-cells from high-glucose-induced cell death by reducing the AGTR1 pathway. |
format | Online Article Text |
id | pubmed-5709427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57094272017-12-06 Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose Kooptiwut, Suwattanee Wanchai, Keerati Semprasert, Namoiy Srisawat, Chatchawan Yenchitsomanus, Pa-thai Sci Rep Article Chronic exposure of pancreatic β-cells to high glucose levels results in β-cell dysfunction and death. These effects can be protected by estrogen. The local pancreatic renin-angiotensin system (RAS) has been shown as a novel pathological pathway of high-glucose-induced cell death. The effect of estrogen on pancreatic RAS is still unknown. This study examines whether estrogen protects against pancreatic β-cell death caused by glucotoxicity via a decrease in the pancreatic β-cell RAS pathway. When INS-1 cells were cultured in a high glucose medium, cell death was significantly higher than when the cells were cultured in a basal glucose medium; similarly, there were also higher levels of AGTR1 and p47 (ph) ° (x) mRNA, and protein expression. Moreover, the addition of 10(−8) M 17β-estradiol to INS-1 cells cultured in a high glucose medium markedly reduced cell death, AGTR1 and p47 (ph) ° (x) mRNA levels, and protein expression. Similar results were demonstrated in the pancreatic islets. The presence of 10(−8) M 17β-estradiol, losartan, or a combination of both, in a high glucose medium had similar levels of reduction of p47 (ph) ° (x) mRNA and protein expression, compared with those cultured in high glucose. Taken together, estrogen protected pancreatic β-cells from high-glucose-induced cell death by reducing the AGTR1 pathway. Nature Publishing Group UK 2017-11-30 /pmc/articles/PMC5709427/ /pubmed/29192236 http://dx.doi.org/10.1038/s41598-017-15237-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kooptiwut, Suwattanee Wanchai, Keerati Semprasert, Namoiy Srisawat, Chatchawan Yenchitsomanus, Pa-thai Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose |
title | Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose |
title_full | Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose |
title_fullStr | Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose |
title_full_unstemmed | Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose |
title_short | Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose |
title_sort | estrogen attenuates agtr1 expression to reduce pancreatic β-cell death from high glucose |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5709427/ https://www.ncbi.nlm.nih.gov/pubmed/29192236 http://dx.doi.org/10.1038/s41598-017-15237-4 |
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