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Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3
Nitidine chloride (NC) is a natural alkaloid compound derived from the plant Zanthoxylum nitidum and is known for its therapeutic anticancer potential. In this study, we investigated the effects of NC on growth and signaling pathways in human oral cancer cell lines and a tumor xenograft model. The a...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5710925/ https://www.ncbi.nlm.nih.gov/pubmed/29207645 http://dx.doi.org/10.18632/oncotarget.20444 |
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author | Kim, Lee-Han Khadka, Sachita Shin, Ji-Ae Jung, Ji-Youn Ryu, Mi-Heon Yu, Hyun-Ju Lee, Hae Nim Jang, Boonsil Yang, In-Hyoung Won, Dong-Hoon Kwon, Hye-Jeong Jeong, Joseph H. Hong, Seong Doo Cho, Nam-Pyo Cho, Sung-Dae |
author_facet | Kim, Lee-Han Khadka, Sachita Shin, Ji-Ae Jung, Ji-Youn Ryu, Mi-Heon Yu, Hyun-Ju Lee, Hae Nim Jang, Boonsil Yang, In-Hyoung Won, Dong-Hoon Kwon, Hye-Jeong Jeong, Joseph H. Hong, Seong Doo Cho, Nam-Pyo Cho, Sung-Dae |
author_sort | Kim, Lee-Han |
collection | PubMed |
description | Nitidine chloride (NC) is a natural alkaloid compound derived from the plant Zanthoxylum nitidum and is known for its therapeutic anticancer potential. In this study, we investigated the effects of NC on growth and signaling pathways in human oral cancer cell lines and a tumor xenograft model. The apoptotic effects and related molecular targets of NC on human oral cancer were investigated using trypan blue exclusion assay, DAPI staining, Live/Dead assay, Western blotting, Immunohistochemistry/Immunofluorescence and a nude mouse tumor xenograft. NC decreased cell viability in both HSC3 and HSC4 cell lines; further analysis demonstrated that cell viability was reduced via apoptosis. STAT3 was hyper-phosphorylated in human oral squamous cell carcinoma (OSCC) compared with normal oral mucosa (NOM) and dephosphorylation of STAT3 by the potent STAT3 inhibitor, cryptotanshinone or NC decreased cell viability and induced apoptosis. NC also suppressed cell viability and induced apoptosis accompanied by dephosphorylating STAT3 in four other oral cancer cell lines. In a tumor xenograft model bearing HSC3 cell tumors, NC suppressed tumor growth and induced apoptosis by regulating STAT3 signaling without liver or kidney toxicity. Our findings suggest that NC is a promising chemotherapeutic candidate against human oral cancer. |
format | Online Article Text |
id | pubmed-5710925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57109252017-12-04 Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3 Kim, Lee-Han Khadka, Sachita Shin, Ji-Ae Jung, Ji-Youn Ryu, Mi-Heon Yu, Hyun-Ju Lee, Hae Nim Jang, Boonsil Yang, In-Hyoung Won, Dong-Hoon Kwon, Hye-Jeong Jeong, Joseph H. Hong, Seong Doo Cho, Nam-Pyo Cho, Sung-Dae Oncotarget Research Paper Nitidine chloride (NC) is a natural alkaloid compound derived from the plant Zanthoxylum nitidum and is known for its therapeutic anticancer potential. In this study, we investigated the effects of NC on growth and signaling pathways in human oral cancer cell lines and a tumor xenograft model. The apoptotic effects and related molecular targets of NC on human oral cancer were investigated using trypan blue exclusion assay, DAPI staining, Live/Dead assay, Western blotting, Immunohistochemistry/Immunofluorescence and a nude mouse tumor xenograft. NC decreased cell viability in both HSC3 and HSC4 cell lines; further analysis demonstrated that cell viability was reduced via apoptosis. STAT3 was hyper-phosphorylated in human oral squamous cell carcinoma (OSCC) compared with normal oral mucosa (NOM) and dephosphorylation of STAT3 by the potent STAT3 inhibitor, cryptotanshinone or NC decreased cell viability and induced apoptosis. NC also suppressed cell viability and induced apoptosis accompanied by dephosphorylating STAT3 in four other oral cancer cell lines. In a tumor xenograft model bearing HSC3 cell tumors, NC suppressed tumor growth and induced apoptosis by regulating STAT3 signaling without liver or kidney toxicity. Our findings suggest that NC is a promising chemotherapeutic candidate against human oral cancer. Impact Journals LLC 2017-08-24 /pmc/articles/PMC5710925/ /pubmed/29207645 http://dx.doi.org/10.18632/oncotarget.20444 Text en Copyright: © 2017 Kim et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Kim, Lee-Han Khadka, Sachita Shin, Ji-Ae Jung, Ji-Youn Ryu, Mi-Heon Yu, Hyun-Ju Lee, Hae Nim Jang, Boonsil Yang, In-Hyoung Won, Dong-Hoon Kwon, Hye-Jeong Jeong, Joseph H. Hong, Seong Doo Cho, Nam-Pyo Cho, Sung-Dae Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3 |
title | Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3 |
title_full | Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3 |
title_fullStr | Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3 |
title_full_unstemmed | Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3 |
title_short | Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3 |
title_sort | nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of stat3 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5710925/ https://www.ncbi.nlm.nih.gov/pubmed/29207645 http://dx.doi.org/10.18632/oncotarget.20444 |
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