Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase

The redesign of enzyme active sites to alter their function or specificity is a difficult yet appealing challenge. Here we used a structure-based design approach to engineer the lactonase SsoPox from Sulfolobus solfataricus into a phosphotriesterase. The five best variants were characterized and the...

Descripción completa

Detalles Bibliográficos
Autores principales: Jacquet, Pauline, Hiblot, Julien, Daudé, David, Bergonzi, Céline, Gotthard, Guillaume, Armstrong, Nicholas, Chabrière, Eric, Elias, Mikael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5711954/
https://www.ncbi.nlm.nih.gov/pubmed/29196634
http://dx.doi.org/10.1038/s41598-017-16841-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5711954/
https://www.ncbi.nlm.nih.gov/pubmed/29196634
http://dx.doi.org/10.1038/s41598-017-16841-0

Ejemplares similares