Cargando…
Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase
The redesign of enzyme active sites to alter their function or specificity is a difficult yet appealing challenge. Here we used a structure-based design approach to engineer the lactonase SsoPox from Sulfolobus solfataricus into a phosphotriesterase. The five best variants were characterized and the...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2017
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5711954/ https://www.ncbi.nlm.nih.gov/pubmed/29196634 http://dx.doi.org/10.1038/s41598-017-16841-0 |