Thiol-linked alkylation of RNA to assess expression dynamics

Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, processing and decay. We developed thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM-s...

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Detalles Bibliográficos
Autores principales: Herzog, Veronika A., Reichholf, Brian, Neumann, Tobias, Rescheneder, Philipp, Bhat, Pooja, Burkard, Thomas R., Wlotzka, Wiebke, von Haeseler, Arndt, Zuber, Johannes, Ameres, Stefan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712218/
https://www.ncbi.nlm.nih.gov/pubmed/28945705
http://dx.doi.org/10.1038/nmeth.4435

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712218/
https://www.ncbi.nlm.nih.gov/pubmed/28945705
http://dx.doi.org/10.1038/nmeth.4435

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