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Thiol-linked alkylation of RNA to assess expression dynamics

Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, processing and decay. We developed thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM-s...

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Detalles Bibliográficos
Autores principales:	Herzog, Veronika A., Reichholf, Brian, Neumann, Tobias, Rescheneder, Philipp, Bhat, Pooja, Burkard, Thomas R., Wlotzka, Wiebke, von Haeseler, Arndt, Zuber, Johannes, Ameres, Stefan L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712218/ https://www.ncbi.nlm.nih.gov/pubmed/28945705 http://dx.doi.org/10.1038/nmeth.4435

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