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Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology

BACKGROUND: Design of experiments is a rapid and cost-effective approach for optimization of recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, w...

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Detalles Bibliográficos
Autores principales: Amiri, Solmaz Agha, Zarei, Najmeh, Enayati, Somayeh, Azizi, Mohammad, Khalaj, Vahid, Shahhosseini, Soraya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5712387/
https://www.ncbi.nlm.nih.gov/pubmed/28689385
http://dx.doi.org/10.22034/ibj.22.1.66