Expression Optimization of Anti-CD22 scFv-Apoptin Fusion Protein Using Experimental Design Methodology

BACKGROUND: Design of experiments is a rapid and cost-effective approach for optimization of recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, we report the effect of different variables on the expression levels of this fusion protein. METHODS: Four variables (cell optical density at induction, IPTG concentration, induction temperature, and induction time) were tested using experimental design. RESULTS: Our findings demonstrated that among the examined variables, only the induction time had a significant positive effect on the protein expression yield. CONCLUSION: Experimental design was successfully applied in this study. The optimized condition obtained in the current study can be applied in future commercial production of this novel fusion protein.