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Generation of Ca(2+)-independent sortase A mutants with enhanced activity for protein and cell surface labeling
Sortase A, a calcium-dependent transpeptidase derived from Staphylococcus aureus, is used in a broad range of applications, such as the conjugation of fluorescent dyes and other moieties to proteins or to the surface of eukaryotic cells. In vivo and cell-based applications of sortase have been somew...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5714338/ https://www.ncbi.nlm.nih.gov/pubmed/29200433 http://dx.doi.org/10.1371/journal.pone.0189068 |