Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy

We describe a method for measuring the single-turnover incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases. Time-lapse total internal reflection fluorescence (TIRF) microscopy is used to directly measure the kinetics of single-base nucleotide incorporation into primed DNA...

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Detalles Bibliográficos
Autores principales: Walsh, Matthew T., Huang, Xiaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716174/
https://www.ncbi.nlm.nih.gov/pubmed/29036327
http://dx.doi.org/10.1093/nar/gkx833

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716174/
https://www.ncbi.nlm.nih.gov/pubmed/29036327
http://dx.doi.org/10.1093/nar/gkx833

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