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Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair

Like tissues, single cells are subjected to continual stresses and damage. As such, cells have a robust wound repair mechanism comprised of dynamic membrane resealing and cortical cytoskeletal remodeling. One group of proteins, the Rho family of small guanosine triphosphatases (GTPases), is critical...

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Detalles Bibliográficos
Autores principales: Nakamura, Mitsutoshi, Verboon, Jeffrey M., Parkhurst, Susan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716286/
https://www.ncbi.nlm.nih.gov/pubmed/28923977
http://dx.doi.org/10.1083/jcb.201704145
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author Nakamura, Mitsutoshi
Verboon, Jeffrey M.
Parkhurst, Susan M.
author_facet Nakamura, Mitsutoshi
Verboon, Jeffrey M.
Parkhurst, Susan M.
author_sort Nakamura, Mitsutoshi
collection PubMed
description Like tissues, single cells are subjected to continual stresses and damage. As such, cells have a robust wound repair mechanism comprised of dynamic membrane resealing and cortical cytoskeletal remodeling. One group of proteins, the Rho family of small guanosine triphosphatases (GTPases), is critical for this actin and myosin cytoskeletal response in which they form distinct dynamic spatial and temporal patterns/arrays surrounding the wound. A key mechanistic question, then, is how these GTPase arrays are formed. Here, we show that in the Drosophila melanogaster cell wound repair model Rho GTPase arrays form in response to prepatterning by Rho guanine nucleotide exchange factors (RhoGEFs), a family of proteins involved in the activation of small GTPases. Furthermore, we show that Annexin B9, a member of a class of proteins associated with the membrane resealing, is involved in an early, Rho family–independent, actin stabilization that is integral to the formation of one RhoGEF array. Thus, Annexin proteins may link membrane resealing to cytoskeletal remodeling processes in single cell wound repair.
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spelling pubmed-57162862018-06-04 Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair Nakamura, Mitsutoshi Verboon, Jeffrey M. Parkhurst, Susan M. J Cell Biol Research Articles Like tissues, single cells are subjected to continual stresses and damage. As such, cells have a robust wound repair mechanism comprised of dynamic membrane resealing and cortical cytoskeletal remodeling. One group of proteins, the Rho family of small guanosine triphosphatases (GTPases), is critical for this actin and myosin cytoskeletal response in which they form distinct dynamic spatial and temporal patterns/arrays surrounding the wound. A key mechanistic question, then, is how these GTPase arrays are formed. Here, we show that in the Drosophila melanogaster cell wound repair model Rho GTPase arrays form in response to prepatterning by Rho guanine nucleotide exchange factors (RhoGEFs), a family of proteins involved in the activation of small GTPases. Furthermore, we show that Annexin B9, a member of a class of proteins associated with the membrane resealing, is involved in an early, Rho family–independent, actin stabilization that is integral to the formation of one RhoGEF array. Thus, Annexin proteins may link membrane resealing to cytoskeletal remodeling processes in single cell wound repair. The Rockefeller University Press 2017-12-04 /pmc/articles/PMC5716286/ /pubmed/28923977 http://dx.doi.org/10.1083/jcb.201704145 Text en © 2017 Nakamura et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Nakamura, Mitsutoshi
Verboon, Jeffrey M.
Parkhurst, Susan M.
Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair
title Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair
title_full Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair
title_fullStr Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair
title_full_unstemmed Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair
title_short Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair
title_sort prepatterning by rhogefs governs rho gtpase spatiotemporal dynamics during wound repair
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716286/
https://www.ncbi.nlm.nih.gov/pubmed/28923977
http://dx.doi.org/10.1083/jcb.201704145
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