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An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns

Rho GTPase-based signaling networks control cellular dynamics by coordinating protrusions and retractions in space and time. Here, we reveal a signaling network that generates pulses and propagating waves of cell contractions. These dynamic patterns emerge via self-organization from an activator–inh...

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Autores principales: Graessl, Melanie, Koch, Johannes, Calderon, Abram, Kamps, Dominic, Banerjee, Soumya, Mazel, Tomáš, Schulze, Nina, Jungkurth, Jana Kathrin, Patwardhan, Rutuja, Solouk, Djamschid, Hampe, Nico, Hoffmann, Bernd, Dehmelt, Leif, Nalbant, Perihan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716289/
https://www.ncbi.nlm.nih.gov/pubmed/29055010
http://dx.doi.org/10.1083/jcb.201706052
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author Graessl, Melanie
Koch, Johannes
Calderon, Abram
Kamps, Dominic
Banerjee, Soumya
Mazel, Tomáš
Schulze, Nina
Jungkurth, Jana Kathrin
Patwardhan, Rutuja
Solouk, Djamschid
Hampe, Nico
Hoffmann, Bernd
Dehmelt, Leif
Nalbant, Perihan
author_facet Graessl, Melanie
Koch, Johannes
Calderon, Abram
Kamps, Dominic
Banerjee, Soumya
Mazel, Tomáš
Schulze, Nina
Jungkurth, Jana Kathrin
Patwardhan, Rutuja
Solouk, Djamschid
Hampe, Nico
Hoffmann, Bernd
Dehmelt, Leif
Nalbant, Perihan
author_sort Graessl, Melanie
collection PubMed
description Rho GTPase-based signaling networks control cellular dynamics by coordinating protrusions and retractions in space and time. Here, we reveal a signaling network that generates pulses and propagating waves of cell contractions. These dynamic patterns emerge via self-organization from an activator–inhibitor network, in which the small GTPase Rho amplifies its activity by recruiting its activator, the guanine nucleotide exchange factor GEF-H1. Rho also inhibits itself by local recruitment of actomyosin and the associated RhoGAP Myo9b. This network structure enables spontaneous, self-limiting patterns of subcellular contractility that can explore mechanical cues in the extracellular environment. Indeed, actomyosin pulse frequency in cells is altered by matrix elasticity, showing that coupling of contractility pulses to environmental deformations modulates network dynamics. Thus, our study reveals a mechanism that integrates intracellular biochemical and extracellular mechanical signals into subcellular activity patterns to control cellular contractility dynamics.
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spelling pubmed-57162892018-06-04 An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns Graessl, Melanie Koch, Johannes Calderon, Abram Kamps, Dominic Banerjee, Soumya Mazel, Tomáš Schulze, Nina Jungkurth, Jana Kathrin Patwardhan, Rutuja Solouk, Djamschid Hampe, Nico Hoffmann, Bernd Dehmelt, Leif Nalbant, Perihan J Cell Biol Research Articles Rho GTPase-based signaling networks control cellular dynamics by coordinating protrusions and retractions in space and time. Here, we reveal a signaling network that generates pulses and propagating waves of cell contractions. These dynamic patterns emerge via self-organization from an activator–inhibitor network, in which the small GTPase Rho amplifies its activity by recruiting its activator, the guanine nucleotide exchange factor GEF-H1. Rho also inhibits itself by local recruitment of actomyosin and the associated RhoGAP Myo9b. This network structure enables spontaneous, self-limiting patterns of subcellular contractility that can explore mechanical cues in the extracellular environment. Indeed, actomyosin pulse frequency in cells is altered by matrix elasticity, showing that coupling of contractility pulses to environmental deformations modulates network dynamics. Thus, our study reveals a mechanism that integrates intracellular biochemical and extracellular mechanical signals into subcellular activity patterns to control cellular contractility dynamics. The Rockefeller University Press 2017-12-04 /pmc/articles/PMC5716289/ /pubmed/29055010 http://dx.doi.org/10.1083/jcb.201706052 Text en © 2017 Graessl et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Graessl, Melanie
Koch, Johannes
Calderon, Abram
Kamps, Dominic
Banerjee, Soumya
Mazel, Tomáš
Schulze, Nina
Jungkurth, Jana Kathrin
Patwardhan, Rutuja
Solouk, Djamschid
Hampe, Nico
Hoffmann, Bernd
Dehmelt, Leif
Nalbant, Perihan
An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns
title An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns
title_full An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns
title_fullStr An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns
title_full_unstemmed An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns
title_short An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns
title_sort excitable rho gtpase signaling network generates dynamic subcellular contraction patterns
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716289/
https://www.ncbi.nlm.nih.gov/pubmed/29055010
http://dx.doi.org/10.1083/jcb.201706052
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