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A simple method to assess group difference in RT-qPCR reference gene selection using GeNorm: The case of the placental sex

Normalization with proper reference genes is a crucial step in obtaining accurate mRNA expression levels in RT-qPCR experiments. GeNorm and NormFinder are two commonly used software packages that help in selecting the best reference genes, based on their expression stability. However, GeNorm does no...

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Detalles Bibliográficos
Autores principales: St-Pierre, Joey, Grégoire, Jean-Charles, Vaillancourt, Cathy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717228/
https://www.ncbi.nlm.nih.gov/pubmed/29209038
http://dx.doi.org/10.1038/s41598-017-16916-y