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Effects of Triton X-100 and PEG on the Catalytic Properties and Thermal Stability of Lipase from Candida Rugosa Free and Immobilized on Glyoxyl-Agarose

BACKGROUND: Candida rugosa Lipase (CRL) shows a very low alkaline stability that comprises its immobilization on glyoxyl-agarose, which requires pH above 10. In this way, an adaptation from the original method was used; an enzyme solution at pH 7 was slowly added at a suspension of glyoxyl-agarose p...

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Detalles Bibliográficos
Autores principales: Perna, Rafael F., Tiosso, Poliana C., Sgobi, Letícia M., Vieira, Angélica M.S., Vieira, Marcelo F., Tardioli, Paulo W., Soares, Cleide M.F., Zanin, Gisella M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721316/
https://www.ncbi.nlm.nih.gov/pubmed/29290831
http://dx.doi.org/10.2174/1874091X01711010066