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Effects of Triton X-100 and PEG on the Catalytic Properties and Thermal Stability of Lipase from Candida Rugosa Free and Immobilized on Glyoxyl-Agarose
BACKGROUND: Candida rugosa Lipase (CRL) shows a very low alkaline stability that comprises its immobilization on glyoxyl-agarose, which requires pH above 10. In this way, an adaptation from the original method was used; an enzyme solution at pH 7 was slowly added at a suspension of glyoxyl-agarose p...
Autores principales: | Perna, Rafael F., Tiosso, Poliana C., Sgobi, Letícia M., Vieira, Angélica M.S., Vieira, Marcelo F., Tardioli, Paulo W., Soares, Cleide M.F., Zanin, Gisella M |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bentham Open
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721316/ https://www.ncbi.nlm.nih.gov/pubmed/29290831 http://dx.doi.org/10.2174/1874091X01711010066 |
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