CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

Cpf1 is a novel class of CRISPR-Cas DNA endonucleases, with a wide range of activity across different eukaryotic systems. Yet, the underlying determinants of this variability are poorly understood. Here, we demonstrate that LbCpf1, but not AsCpf1, ribonucleoprotein complexes allow efficient mutagene...

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Detalles Bibliográficos
Autores principales: Moreno-Mateos, Miguel A., Fernandez, Juan P., Rouet, Romain, Vejnar, Charles E., Lane, Maura A., Mis, Emily, Khokha, Mustafa K., Doudna, Jennifer A., Giraldez, Antonio J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5722943/
https://www.ncbi.nlm.nih.gov/pubmed/29222508
http://dx.doi.org/10.1038/s41467-017-01836-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5722943/
https://www.ncbi.nlm.nih.gov/pubmed/29222508
http://dx.doi.org/10.1038/s41467-017-01836-2

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