Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking

Single‐molecule fluorescence resonance energy transfer (smFRET) is a powerful tool to investigate the dynamics of biomolecular events in real time. However, it requires two fluorophores and can be applied only to dynamics that accompany large changes in distance between the molecules. Herein, we int...

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Detalles Bibliográficos
Autores principales: Kawai, Kiyohiko, Miyata, Takafumi, Shimada, Naohiko, Ito, Syoji, Miyasaka, Hiroshi, Maruyama, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725658/
https://www.ncbi.nlm.nih.gov/pubmed/28990725
http://dx.doi.org/10.1002/anie.201708705
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author Kawai, Kiyohiko
Miyata, Takafumi
Shimada, Naohiko
Ito, Syoji
Miyasaka, Hiroshi
Maruyama, Atsushi
author_facet Kawai, Kiyohiko
Miyata, Takafumi
Shimada, Naohiko
Ito, Syoji
Miyasaka, Hiroshi
Maruyama, Atsushi
author_sort Kawai, Kiyohiko
collection PubMed
description Single‐molecule fluorescence resonance energy transfer (smFRET) is a powerful tool to investigate the dynamics of biomolecular events in real time. However, it requires two fluorophores and can be applied only to dynamics that accompany large changes in distance between the molecules. Herein, we introduce a method for kinetic analysis based on control of fluorescence blinking (KACB), a general approach to investigate the dynamics of biomolecules by using a single fluorophore. By controlling the kinetics of the redox reaction the blinking kinetics or pattern can be controlled to be affected by microenvironmental changes around a fluorophore (rKACB), thereby enabling real‐time single‐molecule measurement of the structure‐changing dynamics of nucleic acids.
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spelling pubmed-57256582017-12-12 Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking Kawai, Kiyohiko Miyata, Takafumi Shimada, Naohiko Ito, Syoji Miyasaka, Hiroshi Maruyama, Atsushi Angew Chem Int Ed Engl Communications Single‐molecule fluorescence resonance energy transfer (smFRET) is a powerful tool to investigate the dynamics of biomolecular events in real time. However, it requires two fluorophores and can be applied only to dynamics that accompany large changes in distance between the molecules. Herein, we introduce a method for kinetic analysis based on control of fluorescence blinking (KACB), a general approach to investigate the dynamics of biomolecules by using a single fluorophore. By controlling the kinetics of the redox reaction the blinking kinetics or pattern can be controlled to be affected by microenvironmental changes around a fluorophore (rKACB), thereby enabling real‐time single‐molecule measurement of the structure‐changing dynamics of nucleic acids. John Wiley and Sons Inc. 2017-10-24 2017-11-27 /pmc/articles/PMC5725658/ /pubmed/28990725 http://dx.doi.org/10.1002/anie.201708705 Text en © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Communications
Kawai, Kiyohiko
Miyata, Takafumi
Shimada, Naohiko
Ito, Syoji
Miyasaka, Hiroshi
Maruyama, Atsushi
Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking
title Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking
title_full Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking
title_fullStr Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking
title_full_unstemmed Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking
title_short Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking
title_sort single‐molecule monitoring of the structural switching dynamics of nucleic acids through controlling fluorescence blinking
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725658/
https://www.ncbi.nlm.nih.gov/pubmed/28990725
http://dx.doi.org/10.1002/anie.201708705
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