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Note on the PCR threshold standard curve
OBJECTIVE: The PCR threshold standard curve is based on an exponential model of the initial phase of a PCR run where template replication efficiency is constant cycle to cycle. As such it requires that a threshold is at a level of amplified template not higher than where replication efficiency falls...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725963/ https://www.ncbi.nlm.nih.gov/pubmed/29228984 http://dx.doi.org/10.1186/s13104-017-3036-4 |