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Note on the PCR threshold standard curve

OBJECTIVE: The PCR threshold standard curve is based on an exponential model of the initial phase of a PCR run where template replication efficiency is constant cycle to cycle. As such it requires that a threshold is at a level of amplified template not higher than where replication efficiency falls...

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Detalles Bibliográficos
Autor principal: Archer, Benedict G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725963/
https://www.ncbi.nlm.nih.gov/pubmed/29228984
http://dx.doi.org/10.1186/s13104-017-3036-4

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