Amplification of nonspecific products in quantitative polymerase chain reactions (qPCR)

Quantitative PCR allows the precise measurement of DNA concentrations and is generally considered to be straightforward and trouble free. However, a survey with 93 validated assays for genes in the Wnt-pathway showed that the amplification of nonspecific products occurs frequently and is unrelated t...

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Detalles Bibliográficos
Autores principales: Ruiz-Villalba, Adrián, van Pelt-Verkuil, Elizabeth, Gunst, Quinn D, Ruijter, Jan M, van den Hoff, Maurice JB
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Original Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727009/
https://www.ncbi.nlm.nih.gov/pubmed/29255685
http://dx.doi.org/10.1016/j.bdq.2017.10.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727009/
https://www.ncbi.nlm.nih.gov/pubmed/29255685
http://dx.doi.org/10.1016/j.bdq.2017.10.001

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