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Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae

Teichoic acid (TA), a crucial cell wall constituent of the pathobiont Streptococcus pneumoniae, is bound to peptidoglycan (wall teichoic acid, WTA) or to membrane glycolipids (lipoteichoic acid, LTA). Both TA polymers share a common precursor synthesis pathway, but differ in the final transfer of th...

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Autores principales: Heß, Nathalie, Waldow, Franziska, Kohler, Thomas P., Rohde, Manfred, Kreikemeyer, Bernd, Gómez-Mejia, Alejandro, Hain, Torsten, Schwudke, Dominik, Vollmer, Waldemar, Hammerschmidt, Sven, Gisch, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727136/
https://www.ncbi.nlm.nih.gov/pubmed/29233962
http://dx.doi.org/10.1038/s41467-017-01720-z
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author Heß, Nathalie
Waldow, Franziska
Kohler, Thomas P.
Rohde, Manfred
Kreikemeyer, Bernd
Gómez-Mejia, Alejandro
Hain, Torsten
Schwudke, Dominik
Vollmer, Waldemar
Hammerschmidt, Sven
Gisch, Nicolas
author_facet Heß, Nathalie
Waldow, Franziska
Kohler, Thomas P.
Rohde, Manfred
Kreikemeyer, Bernd
Gómez-Mejia, Alejandro
Hain, Torsten
Schwudke, Dominik
Vollmer, Waldemar
Hammerschmidt, Sven
Gisch, Nicolas
author_sort Heß, Nathalie
collection PubMed
description Teichoic acid (TA), a crucial cell wall constituent of the pathobiont Streptococcus pneumoniae, is bound to peptidoglycan (wall teichoic acid, WTA) or to membrane glycolipids (lipoteichoic acid, LTA). Both TA polymers share a common precursor synthesis pathway, but differ in the final transfer of the TA chain to either peptidoglycan or a glycolipid. Here, we show that LTA exhibits a different linkage conformation compared to WTA, and identify TacL (previously known as RafX) as a putative lipoteichoic acid ligase required for LTA assembly. Pneumococcal mutants deficient in TacL lack LTA and show attenuated virulence in mouse models of acute pneumonia and systemic infections, although they grow normally in culture. Hence, LTA is important for S. pneumoniae to establish systemic infections, and TacL represents a potential target for antimicrobial drug development.
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spelling pubmed-57271362017-12-14 Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae Heß, Nathalie Waldow, Franziska Kohler, Thomas P. Rohde, Manfred Kreikemeyer, Bernd Gómez-Mejia, Alejandro Hain, Torsten Schwudke, Dominik Vollmer, Waldemar Hammerschmidt, Sven Gisch, Nicolas Nat Commun Article Teichoic acid (TA), a crucial cell wall constituent of the pathobiont Streptococcus pneumoniae, is bound to peptidoglycan (wall teichoic acid, WTA) or to membrane glycolipids (lipoteichoic acid, LTA). Both TA polymers share a common precursor synthesis pathway, but differ in the final transfer of the TA chain to either peptidoglycan or a glycolipid. Here, we show that LTA exhibits a different linkage conformation compared to WTA, and identify TacL (previously known as RafX) as a putative lipoteichoic acid ligase required for LTA assembly. Pneumococcal mutants deficient in TacL lack LTA and show attenuated virulence in mouse models of acute pneumonia and systemic infections, although they grow normally in culture. Hence, LTA is important for S. pneumoniae to establish systemic infections, and TacL represents a potential target for antimicrobial drug development. Nature Publishing Group UK 2017-12-12 /pmc/articles/PMC5727136/ /pubmed/29233962 http://dx.doi.org/10.1038/s41467-017-01720-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Heß, Nathalie
Waldow, Franziska
Kohler, Thomas P.
Rohde, Manfred
Kreikemeyer, Bernd
Gómez-Mejia, Alejandro
Hain, Torsten
Schwudke, Dominik
Vollmer, Waldemar
Hammerschmidt, Sven
Gisch, Nicolas
Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
title Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
title_full Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
title_fullStr Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
title_full_unstemmed Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
title_short Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
title_sort lipoteichoic acid deficiency permits normal growth but impairs virulence of streptococcus pneumoniae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727136/
https://www.ncbi.nlm.nih.gov/pubmed/29233962
http://dx.doi.org/10.1038/s41467-017-01720-z
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