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Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Single-molecule localization microscopy (SMLM) can visualize biological targets on the nanoscale, but complex hardware is required to perform SMLM in thick samples. Here, we combine 3D DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) with spinning disk confocal (SDC) hardware...

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Detalles Bibliográficos
Autores principales: Schueder, Florian, Lara-Gutiérrez, Juanita, Beliveau, Brian J., Saka, Sinem K., Sasaki, Hiroshi M., Woehrstein, Johannes B., Strauss, Maximilian T., Grabmayr, Heinrich, Yin, Peng, Jungmann, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727263/
https://www.ncbi.nlm.nih.gov/pubmed/29233999
http://dx.doi.org/10.1038/s41467-017-02028-8