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Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking

The spatial organization of RNA within cells is a crucial factor influencing a wide range of biological functions throughout all kingdoms of life. However, a general understanding of RNA localization has been hindered by a lack of simple, high-throughput methods for mapping the transcriptomes of sub...

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Detalles Bibliográficos
Autores principales: Kaewsapsak, Pornchai, Shechner, David Michael, Mallard, William, Rinn, John L, Ting, Alice Y
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730372/
https://www.ncbi.nlm.nih.gov/pubmed/29239719
http://dx.doi.org/10.7554/eLife.29224