One-step hot formamide extraction of RNA from Saccharomyces cerevisiae

Current methods for isolating RNA from budding yeast require lengthy and laborious steps such as freezing and heating with phenol, homogenization with glass beads, or enzymatic digestion of the cell wall. Here, extraction with a solution of formamide and EDTA was adapted to isolate RNA from whole ye...

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Detalles Bibliográficos
Autores principales: Shedlovskiy, Daniel, Shcherbik, Natalia, Pestov, Dimitri G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Technical Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731811/
https://www.ncbi.nlm.nih.gov/pubmed/28692404
http://dx.doi.org/10.1080/15476286.2017.1345417

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731811/
https://www.ncbi.nlm.nih.gov/pubmed/28692404
http://dx.doi.org/10.1080/15476286.2017.1345417

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